TUMOR-ASSOCIATED ANTIBODIES IN THE SERUM OF PATIENTS WITH OCULAR MELANOMA .3. IMMUNOPEROXIDASE DETECTION

被引:9
作者
FELBERG, NT
PROLANDAZURI, JM
SHIELDS, JA
FEDERMAN, JL
机构
[1] WILLS EYE HOSP,RETINA RES LAB,PHILADELPHIA,PA 19130
[2] WILLS EYE HOSP,RETINA SERV,ONCOL UNIT,PHILADELPHIA,PA 19130
[3] WILLS EYE HOSP,RES INST,PHILADELPHIA,PA 19130
关键词
D O I
10.1001/archopht.1979.01020010108002
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The indirect immunofluorescent and indirect immunoperoxidase methods to detect tumor-associated antibodies (TAAs) in the plasma of patients with ocular melanomas were examined with commercially available conjugates. Testing indicated comparable titers and staining between the two techniques using snap-frozen uveal melanoma cells. With the indirect immunoperoxidase method it was found that 78% (57) of 73 patients with uveal melanoma had TAAs, while 24% (25) of 106 control subjects were positive for TAAs (P <.001). Seventy-eight percent (11) of 14 patients with metastases to the choroid also had TAAs using snap-frozen cells from choroidal melanoma tumors, which indicates that it was not possible to distinguish this group of patients from those patients with primary uveal melanoma. Tumor-associated antibodies have not been correlated to various prognostic indicators, but serial testing after therapy may show the biologic meaning of such antibodies in patients with ocular melanoma. © 1979, American Medical Association. All rights reserved.
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收藏
页码:256 / 259
页数:4
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