ANCHORAGE-DEPENDENT MAMMALIAN-CELL CULTURE USING POLYURETHANE FOAM AS A NEW SUBSTRATUM FOR CELL ATTACHMENT

被引：33

作者：

MATSUSHITA, T ^{[1
]}

KETAYAMA, M ^{[1
]}

KAMIHATA, K ^{[1
]}

FUNATSU, K ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC ENGN,DEPT CHEM ENGN,6-10-1 HAKOZAKI,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1990年 / 33卷 / 03期

关键词：

D O I：

10.1007/BF00164523

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Anchorage-dependent mammalian cells were cultivated at high cell density in a novel culture system using polyurethane foam (PUF) as a substratum for cell attachment. PUF has a macroporous structure giving a high surface area to volume ratio. Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of PUF and grew to a high cell density (1.04 × 108 cells/ cm3 PUF and 3.5 × 107 cells/ cm3 PUF, respectively) in PUF stationary cultures. In addition, we have designed a PUF-particle packed-bed culture system for high density mass cell culture. A maximum cell density of 2.4 × 107 cells/cm3 culture vessel volume was obtained in a packed-bed culture of Vero cells. © 1990 Springer-Verlag.

引用

页码：287 / 290

页数：4

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