CHEMILUMINESCENT DETERMINATION OF ASCORBIC-ACID IN JUICES

被引：20

作者：

KIM, JM

HUANG, Y

SCHMID, RD

机构：

[1] Division of Enzyme Technology, Gesellschaft für Biotechnologischer Forschung mbH, 3300 Braunschweig

来源：

ANALYTICAL LETTERS | 1990年 / 23卷 / 12期

关键词：

ASCORBIC ACID; CHEMILUMINESCENCE; LUMINOL; LIGHT SUPPRESSION;

D O I：

10.1080/00032719008052564

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly sensitive and rapid chemiluminescent assay method for ascorbic acid was developed. The stopped flow analysis method was applied to the measurement of ascorbate concentrations using 10 or 50-mu-M H2O2, 0.5 mM luminol and 4 U/ml peroxidase to induce the chemiluminescence. A suppression of the light emission occurred in less than 1 min, and the signal was linearly proportional to the ascorbate concentration from 0.1 to 16-mu-M, with a relative standard deviation of less than 2% over the measurable range (n = 4). This suppression of light emission by ascorbate was completely returned to its original unimpeded level of light emission by stopping the flow for several seconds, while suppression by sulfite was not reversible. The method has proven to be good for ascorbate analysis in various juices and wines.

引用

页码：2273 / 2282

页数：10

共 10 条

[1]

CARVALHO LB, 1989, PROCESS BIOCHEM, P52

[2]

Farmer CJ, 1936, P SOC EXP BIOL MED, V34, P146

[3]

GARY H, 1986, METHOD ENZYMOL, V133, P331

[4] REDUCTION OF A NEW WATER-SOLUBLE TETRAZOLIUM SALT BY STEROIDS AND ASCORBIC-ACID [J].