A NOVEL METHOD FOR SPECIFIC VISUALIZATION OF SERUM-ALBUMIN IN POLYACRYLAMIDE GELS BY IODINE STAINING

被引：6

作者：

LEE, KK ^{[1
]}

ELLIS, AE ^{[1
]}

机构：

[1] DAFS MARINE LAB,VICTORIA RD,ABERDEEN AB9 8DB,SCOTLAND

来源：

ELECTROPHORESIS | 1991年 / 12卷 / 05期

关键词：

D O I：

10.1002/elps.1150120513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine serum, bovine serum albumins (delipidated or globulin free or Fraction V), rabbit serum, rabbit serum albumin, Atlantic salmon serum, purified Atlantic salmon serum albumin, human plasma-alpha-2-macroglobulin, hemocyanin, trypsin inhibitor, bovine transferrin and bovine lactoferrin were examined by a novel method for specific visualisation of albumins. In native polyacrylamide gel electrophoresis the albumins were visualised by iodine staining as a transparent spot against a brown background whilst the other proteins could not be visualised. It is suggested that the brown background was due to penetration of the gel by iodine while the chemical binding of iodine by albumin produced a decolourisation reaction. This novel method provides a fast and simple approach to identifying serum albumin in polyacrylamide gels.

引用

页码：382 / 383

页数：2

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