CHARACTERIZATION OF BPV-LIKE DNA IN EQUINE SARCOIDS

被引:85
作者
ANGELOS, JA [1 ]
MARTI, E [1 ]
LAZARY, S [1 ]
CARMICHAEL, LE [1 ]
机构
[1] UNIV BERN,INST ANIM BREEDING,CH-3000 BERN,SWITZERLAND
关键词
D O I
10.1007/BF01314326
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The DNA from equine sarcoid samples from New York State and Switzerland was isolated and probed with bovine papillomavirus type 1 (BPV-1) to determine if BPV genomes were present. Twelve of 13 sarcoids from New York State and 17/20 sarcoids from Switzerland contained DNA that hybridized to the BPV-1 probe. Restriction enzyme analysis of the positive samples demonstrated restriction fragment profiles characteristic of BPV-1 in 22 sarcoids and restriction fragment profiles characteristic of bovine papillomavirus type 2 (BPV-2) in 7 sarcoids. In addition, three tissues histologically diagnosed as pyogranulomatous dermatitis, fibropapilloma, and fibrosarcoma contained BPV-like DNA. Tissues with BPV-1-like and BPV-2-like DNA contained an average of 285.7 (21 to 808) and 125.8 (2 to 762) BPV-like genomes per cell, respectively. Minor differences in the restriction fragment profiles of the BPV-like DNA and evidence for partial BPV-like genomes were found in some sarcoids. BPV-like DNA was not detected in lymphocyte DNA from sarcoid-affected horses. These results confirm previous observations and support the hypothesis that bovine papillomavirus, or a very similar virus, is linked to the cause of equine sarcoid.
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页码:95 / 109
页数:15
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