THE EFFECT OF SURFACE HYDROPHOBICITY AND PROTEIN-CONCENTRATION ON BUFFER CRYSTALLIZATION AND ELISA ABSORBENCY

被引:5
作者
GEDDES, NJ
FURLONG, DN
ZIENTEK, P
THAN, KA
EDGAR, JA
机构
[1] CSIRO,DIV CHEM & POLYMERS,CLAYTON,VIC 3168,AUSTRALIA
[2] CSIRO,DIV ANIM SCI,PARKVILLE,VIC 3052,AUSTRALIA
关键词
D O I
10.1006/jcis.1993.1212
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The crystallization of sodium carbonate buffer from a protein/buffer solution was found to be independent of the surface hydrophobicity when an absorbed protein layer was present. By contrast, the form of the carbonate crystallites was dependent on the protein concentration in solution, ELISA tests on the absorbed protein layer showed that the IgG component of the protein film was more enzymatically active on hydrophilic surfaces. © 1993 Academic Press Limited.
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页码:491 / 497
页数:7
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