OZONE INDUCTION AND PURIFICATION OF SPRUCE CINNAMYL ALCOHOL-DEHYDROGENASE

被引：56

作者：

GALLIANO, H ^{[1
]}

HELLER, W ^{[1
]}

SANDERMANN, H ^{[1
]}

机构：

[1] GSF FORSCHUNGSZENTRUM UMWELT & GESUNDHEIT GMBH,INST BIOCHEM PFLANZENPATHOL,W-8042 NEUHERBERG,GERMANY

来源：

PHYTOCHEMISTRY | 1993年 / 32卷 / 03期

关键词：

PICEA-ABIES; PINACEAE; SPRUCE; CINNAMYL ALCOHOL DEHYDROGENASE; LIGNIN BIOSYNTHESIS; OZONE;

D O I：

10.1016/S0031-9422(00)95136-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spruce needle cinnamyl alcohol dehydrogenase (CAD) was induced in seedlings and four-year-old trees two-to-four-fold by ozone concentrations that did not lead to visible damage. CAD thus acted as an early biochemical ozone marker. A highly purified CAD preparation consisting of two approximately 42 000 M(r) subunits was prepared from spruce cell cultures. A polyclonal antibody was strongly inhibitory. The enzyme had high specificity for coniferyl alcohol and NADP+, and was also detected in spruce seedlings and spruce cambial sap.

引用

页码：557 / 563

页数：7

共 30 条

[1] BOWLES DJ, 1990, ANNU REV BIOCHEM, V59, P873, DOI 10.1146/annurev.bi.59.070190.004301
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] CAMPBELL MM, 1992, PLANTA, V186, P409, DOI 10.1007/BF00195322
[4] CAMPBELL MM, 1992, PHYTOCHEMISTRY, V31, P737, DOI 10.1016/0031-9422(92)80004-X
[5] Dixon R A, 1990, Adv Genet, V28, P165
[6] PHENYLPROPANOID METABOLISM IN SUSPENSION-CULTURES OF VANILLA-PLANIFOLIA .1. INFLUENCE OF GROWTH-REGULATORS AND AN ELICITOR ON PHENYLPROPANOID METABOLISM IN SUSPENSION-CULTURES OF VANILLA-PLANIFOLIA
FUNK, C
BRODELIUS, P
[J]. PHYTOCHEMISTRY, 1990, 29 (03) : 845 - 848
[7] RAPID INDUCTION BY FUNGAL ELICITOR OF THE SYNTHESIS OF CINNAMYL-ALCOHOL DEHYDROGENASE, A SPECIFIC ENZYME OF LIGNIN SYNTHESIS
GRAND, C
SARNI, F
LAMB, CJ
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 169 (01): : 73 - 77
[8] Grisebach H., 1981, The biochemistry of plants. A comprehensive treatise. Volume 7. Secondary plant products., P457
[9] BIOCHEMICAL RESPONSE OF NORWAY SPRUCE (PICEA-ABIES (L) KARST) TOWARDS 14-MONTH EXPOSURE TO OZONE AND ACID MIST .1. EFFECTS ON POLYPHENOL AND MONOTERPENE METABOLISM
HELLER, W
ROSEMANN, D
OSSWALD, WF
BENZ, B
SCHONWITZ, R
LOHWASSER, K
KLOOS, M
SANDERMANN, H
[J]. ENVIRONMENTAL POLLUTION, 1990, 64 (3-4) : 353 - 366
[10] HELLER W, 1990, INT C ACIDIC DEPOSIT

← 1 2 3 →