COMPARATIVE VOLTAMMETRIC STUDY OF 2,4-DINITROPHENOL (DNP), ALBUMIN AND DNP-ALBUMIN AS AN ANALYTICAL APPROACH TO THE USE OF DNP AS A UNIVERSAL LABEL IN IMMUNOELECTROCHEMICAL ASSAYS

被引：8

作者：

COSTAGARCIA, A

FERNANDEZABEDUL, MT

TUNONBLANCO, P

机构：

[1] Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Asturias

来源：

TALANTA | 1994年 / 41卷 / 07期

关键词：

D O I：

10.1016/0039-9140(94)80091-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A comparative electrochemical study of 2,4-dinitrophenol (DNP), albumin and DNP-albumin has been carried out at a hanging mercury drop electrode, in order to use DNP as a universal label in immunoelectrical assays. Several electrochemical techniques have been used. Differential pulse voltammetry has proved to be the most suitable. Wide dynamic linear ranges (more than three orders of magnitude for DNP-albumin) and low detection limits have been achieved (5 x 10(-10) M, 2 x 10(-10) M, 3 x 10(-12) M for DNP, albumin and DNP-albumin, respectively). Good reproducibility has been obtained in all cases (R.S.D. < 2.2%).

引用

页码：1191 / 1200

页数：10

共 31 条

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