MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE PROTYROSINASE GENE, MELO, FROM ASPERGILLUS-ORYZAE AND EXPRESSION OF THE GENE IN YEAST-CELLS

被引：41

作者：

FUJITA, Y ^{[1
]}

URAGA, Y ^{[1
]}

ICHISIMA, E ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,DEPT APPL BIOL CHEM,MOLEC ENZYMOL LAB,AOBA KU,SENDAI,MIYAGI 981,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1261卷 / 01期

关键词：

CDNA SEQUENCE; GENE; GENOMIC DNA; PROTYROSINASE; TYROSINASE; (ASPERGILLUS ORYZAE);

D O I：

10.1016/0167-4781(95)00011-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coding region of the protyrosinase gene, melO, from Aspergillus oryzae occupies of 1671 base pairs of the genomic DNA and is separated into two exons by one intron. The full-length cDNA of the melO gene was cloned. Analysis of the 1617 base pairs nucleotide sequence revealed a single open reading frame coding 539 amino acid residues. The cDNA has been expressed in yeast cells. The predicted protein product derived from the melO gene is identified by Western blotting and activity determination. The predicted amino acid sequence of the gene product was compared with that of Neurospora crassa lyrosinase. A coupled pair of three histidine residues in the tyrosinase was assumed to correspond to Cu(II) ligands in the homologous tyrosinases from Streptomyces glaucescens and Homo sapiens.

引用

页码：151 / 154

页数：4

共 20 条

[1]

BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025

[2] 3.2 A STRUCTURE OF THE COPPER-CONTAINING, OXYGEN-CARRYING PROTEIN PANULIRUS-INTERRUPTUS HEMOCYANIN [J].

GAYKEMA, WPJ ;

HOL, WGJ ;

VEREIJKEN, JM ;

SOETER, NM ;

BAK, HJ ;

BEINTEMA, JJ .

NATURE, 1984, 309 (5963) :23-29

[3]

HUBER M, 1985, BICHEMISTRY, V24, P6033

[4] MULTIPLE FORMS OF PROTYROSINASE FROM ASPERGILLUS-ORYZAE AND THEIR MODE OF ACTIVATION AT PH 3.0 [J].

ICHISHIMA, E ;

MAEBA, H ;

AMIKURA, T ;

SAKATA, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 786 (1-2) :25-31

[5]

Ichishima E., 1986, NIPPON JOZO GAKKAISH, V81, P756

[6]

Ichishima E., 1986, NIPPON JOZO GAKKAISH, V81, P844

[7] HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

INOUE, H ;

NOJIMA, H ;

OKAYAMA, H .

GENE, 1990, 96 (01) :23-28

[8]

KNOWN BS, 1987, P NATL ACAD SCI USA, V84, P7473

[9] COMPILATION AND ANALYSIS OF SEQUENCES UPSTREAM FROM THE TRANSLATIONAL START SITE IN EUKARYOTIC MESSENGER-RNAS [J].

KOZAK, M .

NUCLEIC ACIDS RESEARCH, 1984, 12 (02) :857-872

[10]

KUPPER U, 1989, J BIOL CHEM, V264, P17250

← 1 2 →