A FIELD-EVALUATION OF CYTOCHROME-P4501A AS A BIOMARKER OF CONTAMINANT EXPOSURE IN 3 SPECIES OF FLATFISH

被引:102
作者
COLLIER, TK [1 ]
ANULACION, BF [1 ]
STEIN, JE [1 ]
GOKSOYR, A [1 ]
VARANASI, U [1 ]
机构
[1] UNIV BERGEN, MARINE MOLEC BIOL LAB, HIB, N-5020 BERGEN, NORWAY
关键词
CYP1A; FISH; IMMUNOASSAY; CONTAMINANTS; BIOMARKER;
D O I
10.1002/etc.5620140116
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A study was conducted over the course of a year to determine the induction of hepatic cytochrome P4501A (CYP1A) in three species of benthic fish collected from a contaminated site compared to fish sampled from a less-contaminated site. Juvenile fish were used to minimize effects of reproductive status and migration. CYP1A was determined by two catalytic assays [aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (EROD)I and by an immunoassay (ELISA) utilizing polyclonal antibodies raised against purified CYP1A from cod. AHH activities were measured by a standard method (AHH(std)) and by two variations of the standard method. All three primary CYP1A measures (AHH(std), EROD, and ELISA) showed consistent between-site differences, indicating that induction of CYP1A can be a reliable biomarker of contaminant exposure in fish if appropriate biological variables are controlled for in field studies. Multiple ANOVA demonstrated that the AHH(std) and ELISA data showed less variability due to species or temporal differences, and less unexplained variability, compared to the data from the EROD assay or either variation of the AHH assay. For all measures, variability associated with site differences far outweighed species or temporal variability. Immunoassay, while less sensitive than the AHH(std) assay, is nonetheless recommended to be used in conjunction with catalytic assays because of the potential for samples to lose catalytic activity if not handled properly. The current results suggest that the lower noncontaminant-related variability of AHH(std) makes this CYP1A measure potentially more useful for monitoring programs in which analysis of trends is a primary goal.
引用
收藏
页码:143 / 152
页数:10
相关论文
共 47 条
  • [1] REGULATION OF THE CYTOCHROME-P450 ENZYME-SYSTEM IN FISH
    ANDERSSON, T
    FORLIN, L
    [J]. AQUATIC TOXICOLOGY, 1992, 24 (1-2) : 1 - 19
  • [2] DIETARY-LIPID AS A FACTOR MODULATING XENOBIOTIC METABOLISM IN CHANNEL CATFISH (ICTALURUS-PUNCTATUS)
    ANKLEY, GT
    BLAZER, VS
    PLAKAS, SM
    REINERT, RE
    [J]. CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 1989, 46 (07) : 1141 - 1146
  • [3] Arkoosh M.R., 1991, Fish & Shellfish Immunology, V1, P261, DOI 10.1016/S1050-4648(05)80065-8
  • [4] BILE FLUORESCENCE AND SOME EARLY BIOLOGICAL EFFECTS IN FISH AS INDICATORS OF POLLUTION BY XENOBIOTICS
    BRITVIC, S
    LUCIC, D
    KURELEC, B
    [J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1993, 12 (04) : 765 - 773
  • [5] INDUCIBILITY OF SPAWNING AND REPRODUCTIVE SUCCESS OF FEMALE ENGLISH SOLE (PAROPHRYS-VETULUS) FROM URBAN AND NONURBAN AREAS OF PUGET SOUND, WASHINGTON
    CASILLAS, E
    MISITANO, D
    JOHNSON, LL
    RHODES, LD
    COLLIER, TK
    STEIN, JE
    MCCAIN, BB
    VARANASI, U
    [J]. MARINE ENVIRONMENTAL RESEARCH, 1991, 31 (02) : 99 - 122
  • [6] USING CYTOCHROME-P450 TO MONITOR THE AQUATIC ENVIRONMENT - INITIAL RESULTS FROM REGIONAL AND NATIONAL SURVEYS
    COLLIER, TK
    CONNOR, SD
    EBERHART, BTL
    ANULACION, BF
    GOKSOYR, A
    VARANASI, U
    [J]. MARINE ENVIRONMENTAL RESEARCH, 1992, 34 (1-4) : 195 - 199
  • [7] XENOBIOTIC METABOLIZING ENZYMES IN SPAWNING ENGLISH SOLE (PAROPHRYS-VETULUS) EXPOSED TO ORGANIC-SOLVENT EXTRACTS OF MARINE-SEDIMENTS FROM CONTAMINATED AND REFERENCE AREAS
    COLLIER, TK
    STEIN, JE
    WALLACE, RJ
    VARANASI, U
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1986, 84 (02): : 291 - 298
  • [8] HEPATIC XENOBIOTIC METABOLIZING ENZYMES IN 2 SPECIES OF BENTHIC FISH SHOWING DIFFERENT PREVALENCES OF CONTAMINANT-ASSOCIATED LIVER NEOPLASMS
    COLLIER, TK
    SINGH, SV
    AWASTHI, YC
    VARANASI, U
    [J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 1992, 113 (02) : 319 - 324
  • [9] HEPATIC ACTIVITIES OF XENOBIOTIC METABOLIZING ENZYMES AND BILIARY LEVELS OF XENOBIOTICS IN ENGLISH SOLE (PAROPHRYS-VETULUS) EXPOSED TO ENVIRONMENTAL CONTAMINANTS
    COLLIER, TK
    VARANASI, U
    [J]. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1991, 20 (04) : 462 - 473
  • [10] FIELD STUDIES OF REPRODUCTIVE SUCCESS AND BIOINDICATORS OF MATERNAL CONTAMINANT EXPOSURE IN ENGLISH SOLE (PAROPHRYS-VETULUS)
    COLLIER, TK
    STEIN, JE
    SANBORN, HR
    HOM, T
    MYERS, MS
    VARANASI, U
    [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 1992, 116 (1-2) : 169 - 185