BETA-D-GALACTOSIDASE ACTIVITY OF VIABLE, NON-CULTURABLE COLIFORM BACTERIA IN MARINE WATERS

被引:37
作者
DAVIES, CM
APTE, SC
PETERSON, SM
机构
[1] Centre for Advanced Analytical Chemistry, CSIRO, Division of Coal and Energy Technology, Menai, New South Wales
关键词
D O I
10.1111/j.1472-765X.1995.tb01016.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The beta-D-galactosidase activity of viable but non-culturable (vnc) Escherichia coli cells in seawater was investigated using a rapid fluorimetric enzyme assay. Results from microcosm studies showed that loss of culturability did not necessarily result in loss of the ability to produce the galactosidase enzyme. Even when no culturable cells were detected, a positive enzyme assay response was observed and the activity of the inducible enzyme over time more closely reflected the number of vnc cells present.
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页码:99 / 102
页数:4
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