ASSOCIATION AND COEXPRESSION OF FATTY-ACID-BINDING PROTEIN AND GLYCOPROTEIN CD36 IN THE BOVINE MAMMARY-GLAND

被引:145
作者
SPITSBERG, VL
MATITASHVILI, E
GOREWIT, RC
机构
[1] Department of Animal Science, Cornell University, Ithaca
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 230卷 / 03期
关键词
FATTY-ACID-BINDING PROTEIN (FABP); MAMMARY-DERIVED GROWTH INHIBITOR (MDGI); GLYCOPROTEIN CD36; MAMMARY GLAND EPITHELIAL CELL; MILK FAT GLOBULE MEMBRANE (MFGM);
D O I
10.1111/j.1432-1033.1995.tb20630.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The involvement of glycoprotein CD36 and fatty-acid-binding protein (FABP) in cellular growth, differentiation, lipid transport and metabolism led us to examine the possible biochemical and physiological relationship(s) between these two proteins. We investigated three aspects of this relationship. We first attempted to identify any physical complex formed between CD36 and FABP in bovine milk fat globule membranes. These membranes are the product of mammary gland secretory epithelial cells. The second aspect studied was the effect of synthetic peptide analogs to the C-terminus (amino acid residues 121-131) of bovine mammary gland FABP on cell proliferation, as a result of the interaction of these peptides with the ectodomain of CD36. Finally, mammary gland CD36 and FABP coexpression was defined at different stages of lactation and during involution. Immunoprecipitation, Western immunoblotting with anti-FABP and anti-CD36, Northern-blot analysis and a mammary epithelial cell proliferation assay demonstrated that: (a) bovine milk fat globule membranes contain the complex of CD36 and FABP, and that this complex is, most likely, formed as a result of FABP binding to the cytoplasmic segments of CD36; (b) synthetic analog of the C-terminus of FABP with the sequence Val-Thr-Cys, identical to the sequence found in the CD36-binding domain of thrombospondin, was a more potent inhibitor of bovine mammary gland epithelial cell proliferation than a synthetic peptide with the Val-Cys-Thr sequence; (c) the expression of FABP and CD36 is related to the state of mammary cell differentiation, since it reaches its maximum during lactation and declines during the involutionary period.
引用
收藏
页码:872 / 878
页数:7
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