STUDIES ON ANTIGENIC ACTIVITIES OF YEASTS .2. ISOLATION AND INHIBITION ASSAY OF OLIGOSACCHARIDES FROM ACETOLYSATE OF MANNAN OF CANDIDA ALBICANS

In order to determine the antigenic determinant groups of the mannan of Candida albicans by the precipitation‐inhibition test, several oligosaccharides were prepared by acetolysis of the polysaccharide. The manno‐oligosaccharides, from biose to heptaose were separated by a charcoal‐Celite chromatography and a subsequent cellulose column chromatography. The oligosaccharides thus separated were examined on the degrees of polymerization and the mode of the linkages, and evidence was obtained that the biose and triose were joined through α1→2 linkage only, while the tetraose, pentaose and hexaose contained α1→3 linkage in addition to α1→2 linkages. Heptaose was joined entirely through α1→2 linkage. In the precipitation‐inhibition test, the inhibitory power of the oligosaccharides of acetolysis product was found to be the following order: hexaose>heptaose>pentaose>tetraose>triose>biose, and the amount for the 50% inhibitions were 0.025, 0.09, 0.12, 0.60, 3.96 and 5.84 μmoles respectively. On the other hand, the biose, triose and tetraose, which were isolated from the acid‐hydrolysate of the mannan of Saccharomyces cerevisiae and joined through α1→6 linkage, showed poor or nearly no inhibitory power. The above facts provide an evidence that the consecutive α1→6 linkages were not located in a position that is responsible for antigenic specificity of the mannan of C. albicans. © jointly owned by Japanese society for Bacteriology and Society of Japanese Virologists