HEPARIN-MODIFIED POLYLACTIDE AS BIODEGRADABLE HEMOCOMPATIBLE BIOMATERIAL

被引：10

作者：

HERRMANN, K ^{[1
]}

GROTH, T ^{[1
]}

SEIFERT, B ^{[1
]}

ROMANIUK, P ^{[1
]}

机构：

[1] HUMBOLDT UNIV BERLIN, KLINIKUM CHARITE, BEREICH INTERVENT RADIOL ANGIOL, INST RONTGENDIAGNOST, D-10098 BERLIN, GERMANY

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 1994年 / 5卷 / 9-10期

关键词：

D O I：

10.1007/BF00120365

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Several medical applications may benefit from biodegradable hemocompatible materials. Therefore a commercial biodegradable polylactide (RESOMER(R) R208; Boehringer-lngelheim, Germany) was modified with heparin. The immobilization of heparin was performed by covalent binding with glutaraldehyde as spacer. Reaction conditions were varied to optimize the coupling of heparin. The efficiency of the immobilization and the biological activity of bonded heparin was estimated with a toluidine blue assay and factor Xa assay, respectively. It was shown that an immobilization at ambient temperature and a reaction time of 2 h yielded maximal heparin binding and biological activity. The hemocompatibility of the modified polylactide was tested after blood/material contact with an enzyme immune assay (EIA) for GMP 140, a marker of platelet activation. The investigated materials were compared with polypropylene and Pellethane(R). After modification it was found that high heparin binding and biological activity were correlated with very small activation of platelets.

引用

页码：728 / 731

页数：4

共 20 条

[1]

ALTANKOV G, 1990, HAEMATOLOGIA, V23, P239

[2]

ALTANKOV G, 1988, HAEMATOLOGIA, V21, P99

[3] SURFACE MODIFICATION OF POLYMERIC BIOMATERIALS WITH POLY(ETHYLENE OXIDE), ALBUMIN, AND HEPARIN FOR REDUCED THROMBOGENICITY [J].