A SINGLE-ELEMENT OF THE ELASTASE-I ENHANCER IS SUFFICIENT TO DIRECT TRANSCRIPTION SELECTIVELY TO THE PANCREAS AND GUT

被引:33
作者
ROSE, SD
KRUSE, F
SWIFT, GH
MACDONALD, RJ
HAMMER, RE
机构
[1] UNIV TEXAS,SW MED CTR,DEPT BIOCHEM,DALLAS,TX 75235
[2] UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST,DALLAS,TX 75235
关键词
D O I
10.1128/MCB.14.3.2048
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The elastase I (EI) gene is expressed at high levels in the exocrine pancreas and at lower levels in other regions of the gut. The transcriptional enhancer of the EI gene, from nucleotides -205 to -72, recapitulates the expression of the endogenous gene in transgenic mice; it directs not only pancreatic acinar cell expression of a human growth hormone (hGH) transgene but also expression to the stomach, duodenum, and colon. This pattern of selective expression limited to the gastroenteropancreatic organ system is specified by the A element, one of three functional elements in the EI enhancer. When multimerized, the A element directed expression of a hGH reporter gene selectively to the pancreas, stomach, and intestine in transgenic mice, Immunofluorescent localization of hGH indicated that the A element multimer transgenes were expressed in the acinar cells of the pancreas as well as in Brunner's gland cells of the duodenum. The A element binds a pancreatic acinar cell-specific factor, PTF1. Our results show that while the A element is responsible for directing tissue and cell type specificity, other elements of the enhancer must be involved in the regulation of the level of gene expression.
引用
收藏
页码:2048 / 2057
页数:10
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