SOLUBILIZATION AND PROPERTIES OF A PHOSPHATIDYLETHANOLAMINE-DEPENDENT METHYLTRANSFERASE SYSTEM FOR PHOSPHATIDYLCHOLINE SYNTHESIS FROM MOUSE-LIVER MICROSOMES

被引：42

作者：

TANAKA, Y

DOI, O

AKAMATSU, Y

机构：

[1] Department of Chemistry, National Institute of Health, Shinagawa, Tokyo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 04期

关键词：

D O I：

10.1016/S0006-291X(79)80022-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method was developed for solubilization of a phosphatidylethanolamine-dependent methyltransferase system for phosphatidylcholine synthesis in mouse liver microsomes. A preparation with high specific activity was obtained in good yield by treating 0.3% deoxycholate-treated microsomes with 0.2% Triton X-100. With this preparation, methyl incorporation into phospholipids was activated by phosphatidylethanolamine and its methylated intermediates. The specific activity of the preparation with phosphatidylethanolamine was 4 times that of intact microsomes. The reaction products with the solubilized preparation were phosphatidylcholine and methylated intermediates of phosphatidylethanolamine. © 1979 Academic Press, Inc. All rights of reproduction in any form reserved.

引用

页码：1109 / 1115

页数：7

共 21 条

[1] AMES BN, 1960, J BIOL CHEM, V235, P769
[2] METHYL TRANSFERING ENZYME SYSTEM OF MICROSOMES IN BIOSYNTHESIS OF LECITHIN (PHOSPHATIDYLCHOLINE)
BREMER, J
GREENBERG, DM
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1961, 46 (02) : 205 - &
[3] DOI O, 1973, J BIOCHEM-TOKYO, V74, P667, DOI 10.1093/oxfordjournals.jbchem.a130291
[4] FIGARD PH, 1962, CANCER RES, V22, P361
[5] GIBSON KD, 1961, J BIOL CHEM, V236, P673
[6] MANIPULATION OF PHOSPHOLIPID COMPOSITION OF TISSUE-CULTURE CELLS
GLASER, M
FERGUSON, KA
VAGELOS, PR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (10) : 4072 - 4076
[7] ENZYMATIC-SYNTHESIS AND RAPID TRANSLOCATION OF PHOSPHATIDYLCHOLINE BY 2 METHYLTRANSFERASES IN ERYTHROCYTE-MEMBRANES
HIRATA, F
AXELROD, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (05) : 2348 - 2352
[8] ENZYMATIC METHYLATION OF PHOSPHATIDYLETHANOLAMINE INCREASES ERYTHROCYTE-MEMBRANE FLUIDITY
HIRATA, F
AXELROD, J
[J]. NATURE, 1978, 275 (5677) : 219 - 220
[9] IDENTIFICATION AND PROPERTIES OF 2 METHYLTRANSFERASES IN CONVERSION OF PHOSPHATIDYLETHANOLAMINE TO PHOSPHATIDYLCHOLINE
HIRATA, F
VIVEROS, OH
DILIBERTO, EJ
AXELROD, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (04) : 1718 - 1721
[10] KANESHIRO T, 1964, J BIOL CHEM, V239, P1705

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