INCREASES IN LOCAL CEREBRAL BLOOD-FLOW ASSOCIATED WITH SOMATOSENSORY ACTIVATION ARE NOT MEDIATED BY NO

被引:58
作者
ADACHI, K
TAKAHASHI, S
MELZER, P
CAMPOS, KL
NELSON, T
KENNEDY, C
SOKOLOFF, L
机构
[1] NIMH, CEREBRAL METAB LAB, BETHESDA, MD 20892 USA
[2] NIMH, NEUROCHEM LAB, BETHESDA, MD 20892 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1994年 / 267卷 / 06期
关键词
N-G-NITRO-L-ARGININE METHYL ESTER; WHISKER BARRELS; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; FUNCTIONAL ACTIVATION; BRAIN; IODO[C-14]ANTIPYRINE;
D O I
10.1152/ajpheart.1994.267.6.H2155
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Effects of inhibition of nitric oxide (NO) synthase by N-G-nitro-L-arginine methyl ester (L-NAME) on the increases in local cerebral blood flow (LCBF) produced in the whisker-to-barrel sensory pathway by vibrissal stimulation were studied in conscious rats with the autoradiographic iodo [C-14]antipyrine method. Unilateral whisker stroking increased LCBF in the ipsilateral trigeminal spinal and principal sensory nuclei, contralateral ventral posteromedial thalamic nucleus, and contralateral somatosensory barrel cortex. Intravenous L-NAME (30 mg/kg) lowered baseline LCBF without altering the percent increases due to stimulation. Intracisternal infusions of L-NAME in doses about 10 times the molar content of free arginine in brain inhibited brain NO synthase activity by 88%, but the percent augmentations of LCBF by stimulation remained unchanged. Chronic treatment with L-NAME (50 mg/kg ip twice daily for 4 days) inhibited NO synthase activity in brain by 84% but also failed to reduce the percent increases in LCBF due to stimulation. These results indicate that NO does not mediate the increases in LCBF associated with functional activation.
引用
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页码:H2155 / H2162
页数:8
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