DIETARY MANIPULATION OF LONG-CHAIN POLYUNSATURATED FATTY-ACIDS IN THE RETINA AND BRAIN OF GUINEA-PIGS

被引:35
作者
WEISINGER, HS
VINGRYS, AJ
SINCLAIR, AJ
机构
[1] ROYAL MELBOURNE INST TECHNOL,DEPT FOOD SCI,MELBOURNE,VIC 3001,AUSTRALIA
[2] UNIV MELBOURNE,DEPT OPTOMETRY,PARKVILLE,VIC 3052,AUSTRALIA
关键词
D O I
10.1007/BF02536307
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
High levels of n-6 docosapentaenoic acid (22:5n-6) have been reported in the retina of guinea pigs fed commercially-prepared grain-based rations (commercial diet). In rats and monkeys, high levels of 22:5n-6 are an indicator of n-3 polyunsaturated fatty acid (PUFA) deficiency. We have examined the fatty acid composition of the retina and brain in guinea pigs fed a commercial diet or one of three semi-purified diets containing three different levels of n-3 PUFA. The diets comprised a diet deficient in n-3 PUFA (semi-purified diet containing safflower oil), two diets containing alpha-linolenic acid (standard commercial laboratory diet and semi-purified diet containing canola oil), and a diet containing alpha-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid (DHA) (semi-purified diet containing canola oil, safflower oil, and fish oil). Two groups of guinea pigs were given the diets from day 1 to 4 wk or day 1 to 8 wk, when they were sacrificed and the retinal tissues were extracted and analyzed for PUFA content by gas-liquid chromatography. Fatty acid analyses of the retinal phospholipids of the four-week-old animals revealed that the group fed DHA (from the fish oil) had the highest level of DHA (32%), compared with values of 19 and 13% for the groups fed canola oil diet and commercial diet, respectively, and 2% for the group fed the diet deficient in n-3 PUFA. The levels of 22:5n-6 in the retinal lipids were inversely related to the DHA values, being 0.6, 6.6, 11.4, and 20.6 for the fish oil, canola oil, commercial diet, and safflower oil diet groups, respectively. The long-chain PUFA profiles in the brain phospholipids of the four-week-old group were similar to those from the retina. The retinal PUFA values for the eight-week-old animals were similar to the four-week-old group. The safflower oil diet induced a greater deficit of DHA in retinal lipids than has been reported in rats and monkeys fed similar diets. The guinea pigs fed the commercial diet had retinal and brain PUFA patterns similar to that produced by n-3 PUFA-deficient diets in rats and monkeys. Guinea pigs fed the canola oil diet had significantly greater retinal DHA levels than those fed the commercial diet, but lower than those fed fish oil. The data suggest that the guinea pig has a reduced capacity for DHA synthesis from alpha-linolenic acid as compared with other mammals. Supplementation of guinea pig diets with fish oil produced high retinal and brain DHA levels and prevented the accumulation of 22:5n-6.
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页码:471 / 473
页数:3
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  • [1] Crawford M.A., Casperd N.M., Sinclair A.J., The Long Chain Metabolites of Linoleic and Linolenic Acids in Liver and Brain in Herbivores and Carnivores, Comp. Biochem. Physiol., 54 B, pp. 395-401, (1976)
  • [2] Sinclair A.J., Long Chain Polyunsaturated Fatty Acids in the Mammalian Brain, Proc. Nutr. Soc., 34, pp. 287-291, (1975)
  • [3] Connor W.E., Neuringer M., Reisbick S., Essential Fatty Acids: The Importance of n−3 Fatty Acids in the Retina and Brain, Nutr. Rev., 50, pp. 21-29, (1992)
  • [4] Bourre J.M., Francois M., Youyou A., Dumont O., Piciotti M., Pascal G., Durand G., The Effect of Dietary Alpha-Linolenic Acid on the Composition of Nerve Membranes, Enzymatic Activity, Amplitude of Electrophysiological Parameters, Resistance to Poisons and Performance of Learning Tasks in Rats, J. Nutr., 119, pp. 1880-1892, (1989)
  • [5] Watanabe I., Kato M., Aonuma H., Hashimoto A., Naito Y., Moriuchi A., Okuyama H., Effect of Dietary Alpha-Linolenate/Linoleate Balance on the Lipid Composition and Electroretinographic Responses in Rats, Adv. Biosci., 62, pp. 563-570, (1987)
  • [6] Neuringer M., Connor W.E., Lin D.S., Barstad L., Luck S., Biochemical and Functional Effects of Prenatal and Postnatal Omega-3 Fatty Acid Deficiency on Retina and Brain in Rhesus Monkeys, Proc. Natl. Acad. Sci., 83, pp. 4021-4025, (1986)
  • [7] Leat W.M.F., Curtis R., Millichamp N.J., Cow R.W., Retinal Function in Rats and Guinea Pigs Reared on Diets Low in Essential Fatty Acids and Supplemented with Linoleic or Linolenic Acids, Ann. Nutr. Metab., 30, pp. 166-174, (1986)
  • [8] Sinclair A.J., O'Dea K., Dunstan G., Ireland P.D., Niall M., Effects on Plasma Lipids and Fatty Acid Composition of Very Low Fat Diets Enriched with Fish or Kangaroo Meat, Lipids, 22, pp. 523-529, (1987)
  • [9] Fiennes R.N.T-W., Sinclair A.J., Crawford M.A., Essential Fatty Acid Studies in Primates: Alpha-Linolenic Acid Requirements of Capuchins, J. Med. Primatology, 2, pp. 155-169, (1973)
  • [10] Lin D.S., Anderson G.J., Connor W.E., High Levels of the (n−6) Fatty Acid 4,7,10,13,16-Docosapentaenoic Acid in the Retinas of Rabbits Are Reduced by Feeding Dietary Fish Oil from Birth to Adult Live, J. Nutr., 121, pp. 1924-1931, (1991)