OXIDATIVE DEMETHYLATION OF N-METHYLHYDRAZINES BY RAT LIVER MICROSOMES

被引：42

作者：

WITTKOP, JA

PROUGH, RA

REED, DJ

机构：

[1] Department of Biochemistry and Biophysics, the Radiation Center, Oregon State University, Corvallis

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 134卷 / 02期

关键词：

D O I：

10.1016/0003-9861(69)90289-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Certain N-methylhydrazines were found to be oxidatively demethylated to yield formaldehyde by rat liver microsomal preparations. Substrates for the N-demethylase were found to be monomethylhydrazine, 1,1-dimethylhydrazine, 1,2-dimethylhydrazine, Procarbazine, and the azo derivative of Procarbazine. Oxygen and a NADPH-regenerating system were required for maximum activity. Carbon monoxide inhibited the demethylation reaction but no inhibition was found by SKF 525-A. Phenobarbital or 3-methylcholanthrene pretreatment of the animals caused an enhancement of the demethylase activity. P-450 appears to play a role in the phenobarbital-inducible reaction since treatment of the microsomes with steapsin or deoxycholate which convert P-450 to P-420 inhibited only the induced activity. © 1969.

引用

页码：308 / &

共 35 条

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