ELEVATED CYTOSOLIC CALCIUM IN CEREBROCORTICAL NERVE-TERMINALS OF RATS DURING PROLONGED ETHANOL INGESTION

被引：2

作者：

COLLINS, MA

RAIKOFF, K

机构：

[1] Department of Biochemistry Loyola University, Chicago Stritch School of Medicine Maywood

来源：

LIFE SCIENCES | 1990年 / 47卷 / 14期

关键词：

D O I：

10.1016/0024-3205(90)90214-C

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Increases in cytosolic free calcium concentrations ([Ca++]i) may underlie acute neuronal degeneration during ischemic or anoxic episodes, seizures and excitotoxin treatment. With quin-2 and fura-2 fluorescent probes, we have obtained evidence for elevated [Ca++]i in cerebrocortical terminals of adult rats following chronic consumption of ethanol-containing liquid diets for "neurotoxic" durations. Compared to isocaloric carbohydrate-fed controls, ethanol-fed rats had significantly higher [Ca++]i in P2 synaptosomal fractions after 4 months of diet intake, and in purified cerebrocortical synaptosomes after diet ingestion for 10 months. In addition, [Ca++]i in the synaptosomal fractions of ethanol-fed rats from either exposure time were markedly resistant to K+-dependent potentiation. Persistently increased synaptic [Ca++]i and a blunted response to K+ depolarization following chronic ethanol ingestion lead us to associate impaired Ca++ homeostasis in the neurodegenerative processes of alcoholism. © 1990.

引用

页码：1221 / 1226

页数：6

共 29 条

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