DISTINCT PATHWAYS REGULATE TRANSFORMING GROWTH FACTOR-BETA-1-STIMULATED PROTO-ONCOGENE AND EXTRACELLULAR-MATRIX GENE-EXPRESSION

被引：32

作者：

HOWE, PH ^{[1
]}

CUNNINGHAM, MR ^{[1
]}

LEOF, EB ^{[1
]}

机构：

[1] VANDERBILT UNIV, DEPT CELL BIOL, NASHVILLE, TN 37232 USA

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1990年 / 142卷 / 01期

关键词：

D O I：

10.1002/jcp.1041420106

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effect of pertussis toxin (PT) on transforming growth factor β1 (TGFβl)‐induced proto‐oncogene expression was investigated in AKR‐2B fibroblasts. PT substantially abolished c‐sis and c‐myc mRNA expression following TGFβl stimulation. This inhibitory effect was specific for TGFβ1‐stimulated proto‐oncogene expression and associated with the ADP‐ribosylation of a 41‐kDa substrate. Actinomycin D decay and nuclear run‐on experiments demonstrated that the inhibitory effects of PT are a result of decreased transcriptional activation and not to an increased decay of proto‐oncogene message. PT did not, however, affect TGFβl‐stimulated fibronectin and collagen mRNA accumulation nor did it have any inhibitory effect on TGFβl‐induced morphological transformation. These data indicate that TGFβl‐stimulated gene expression is coupled to multiple pathways distinguished by their sensitivity to PT. Copyright © 1990 Wiley‐Liss, Inc.

引用

页码：39 / 45

页数：7

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