M-SMAL IS AN N4-METHYLCYTOSINE SPECIFIC DNA-METHYLASE

被引：17

作者：

KLIMASAUSKAS, S ^{[1
]}

STEPONAVICIENE, D ^{[1
]}

MANELIENE, Z ^{[1
]}

PETRUSYTE, M ^{[1
]}

BUTKUS, V ^{[1
]}

JANULAITIS, A ^{[1
]}

机构：

[1] INST APPL ENZYMOL,FERMENTU 8,VILNIUS 232028,LITHUANIA,USSR

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 22期

关键词：

D O I：

10.1093/nar/18.22.6607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzymatic activity rendering DNA immune to the action of the Smal restriction endonuclease in the presence of S-adenosyl-L-methionine has been detected in Serratla marcescens Sb. This methylase, M.Smal, modifies the second cytosine residue of the substrate sequence CCCGGG yielding N4-methylcytosine. © 1990 Oxford University Press.

引用

页码：6607 / 6609

页数：3

共 9 条

[1] CLEAVAGE OF METHYLATED CCCGGG SEQUENCES CONTAINING EITHER N4-METHYLCYTOSINE OR 5-METHYLCYTOSINE WITH MSPI, HPAII, SMAI, XMAI AND CFR9I RESTRICTION ENDONUCLEASES [J].

BUTKUS, V ;

PETRAUSKIENE, L ;

MANELIENE, Z ;

KLIMASAUSKAS, S ;

LAUCYS, V ;

JANULAITIS, A .

NUCLEIC ACIDS RESEARCH, 1987, 15 (17) :7091-7102

[2] N4-METHYLCYTOSINE AS A MINOR BASE IN BACTERIAL-DNA [J].

EHRLICH, M ;

WILSON, GG ;

KUO, KC ;

GEHRKE, CW .

JOURNAL OF BACTERIOLOGY, 1987, 169 (03) :939-943

[3] CLONING, CHARACTERIZATION AND HETEROLOGOUS EXPRESSION OF THE SMAI RESTRICTION-MODIFICATION SYSTEM [J].

HEIDMANN, S ;

SEIFERT, W ;

KESSLER, C ;

DOMDEY, H .

NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :9783-9796

[4] SEQUENCE MOTIFS CHARACTERISTIC OF DNA[CYTOSINE-N4]METHYLTRANSFERASES - SIMILARITY TO ADENINE AND CYTOSINE-C5 DNA-METHYLASES [J].

KLIMASAUSKAS, S ;

TIMINSKAS, A ;

MENKEVICIUS, S ;

BUTKIENE, D ;

BUTKUS, V ;

JANULAITIS, A .

NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :9823-9832

[5]

KLIMASAUSKAS S, 1990, EKSPERIMENTINE BIOL, V1, P7

[6]

MULDER C, 1989, NUCLEIC ACIDS RES, V17, pR347

[7]

PETRAUSKIENE LJ, 1986, BIOORG KHIM+, V12, P1597

[8] PREDICTIVE MOTIFS DERIVED FROM CYTOSINE METHYLTRANSFERASES [J].

POSFAI, J ;

BHAGWAT, AS ;

POSFAI, G ;

ROBERTS, RJ .

NUCLEIC ACIDS RESEARCH, 1989, 17 (07) :2421-2435

[9] DETECTION OF METHYLATED SEQUENCES IN EUKARYOTIC DNA WITH THE RESTRICTION ENDONUCLEASES SMAI AND XMAI [J].

YOUSSOUFIAN, H ;

MULDER, C .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 150 (01) :133-136

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