ENHANCED CHEMILUMINESCENCE IN THE OXIDATION OF LUMINOL AND AN ISOLUMINOL CORTISOL CONJUGATE BY HYDROGEN-PEROXIDE IN REVERSED MICELLES

被引：10

作者：

METELITZA, DI

ERYOMIN, AN

SHIBAEV, VA

机构：

[1] Institute of Bioorganic Chemistry, BSSR Academy of Sciences, USSR., Minsk

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1992年 / 7卷 / 01期

关键词：

ENHANCED CHEMILUMINESCENCE; LUMINOL; ISOLUMINOL CONJUGATE; REVERSED MICELLES; CETYLTRIMETHYLAMMONIUM BROMIDE;

D O I：

10.1002/bio.1170070104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemiluminescent oxidation of luminol and an isoluminol cortisol conjugate (ABI-COR) by hydrogen peroxide has been studied in- cetyltrimethylammonium bromide (CTAB) reversed micelles in octane-chloroform (1 : 1). The maximum chemiluminescence intensity of both compounds is dependent on the initial concentrations of the H2O2 and substrates, the pH value of the micelle Polar phase and the H2O/CTAB ratio. The optimum pH ranged from 8.5 to 9.5. Under comparable conditions, the chemiluminescence intensity for luminol was 15-fold higher than for the ABI-COR conjugate. A mechanism of oxidation of the substrates in reversed micelles is proposed and the possible mechanisms of inhibition by the substrate and oxidant is discussed.

引用

页码：21 / 26

页数：6

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