USE OF ANILINE BLUE TO ASSESS CHROMATIN CONDENSATION IN MORPHOLOGICALLY NORMAL SPERMATOZOA IN NORMAL AND INFERTILE MEN

被引：74

作者：

HOFMANN, N

HILSCHER, B

机构：

[1] Andrology of the Heinrich-Heine University Dusseldorf, D 4000 Düsseldorf

[2] Department of Experimental Pathology, Medical Institute of Environmental Hygiene, D-4000 Düsseldorf

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 07期

关键词：

ANILINE BLUE STAINING; CHROMATIN CONDENSATION; SPERM MALFORMATION; ASSISTED FERTILIZATION;

D O I：

10.1093/oxfordjournals.humrep.a137472

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Chromatin condensation is vital for the function of the spermatozoon as the motile carrier of the paternal genome. The degree of condensation can be shown with the aid of acidic aniline blue staining, which is able to discriminate between lysine-rich histones and arginine- and cysteine-rich protamines. Using this technique and employing the Dusseldorf classification of sperm morphology in cases of disturbance of spermatogenesis, it was demonstrated that chromatin condensation is impaired not only in malformed but also in morphologically normal spermatozoa. Among morphologically normal spermatozoa, the percentages of spermatozoa with chromatin condensation disturbances increase in patients with different patterns of sperm malformation, if compared with patients with normozoospermia.

引用

页码：979 / 982

页数：4

共 17 条

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