CONSTRUCTION OF A BROAD-HOST-RANGE NON-MOBILIZABLE STABLE VECTOR CARRYING RP4 PAR-REGION

被引：5

作者：

CROUZET, J

LEVYSCHIL, S

CAUCHOIS, L

CAMERON, B

机构：

[1] Département Biotechnologies, Rhône-Poulenc Rorer S.A., Centre de Recherche de Vitry-Alfortville, Vitry sur Seine

来源：

GENE | 1992年 / 110卷 / 01期

关键词：

RECOMBINANT DNA; PLASMID STABILITY; GRAM-NEGATIVE BACTERIA;

D O I：

10.1016/0378-1119(92)90451-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmid pXL1635 was constructed from the already segregationally stable incP-derived pRK290. Plasmid pXL1635 should be suitable for industrial and environmental uses in Gram- bacteria since (i) it contains the par fragment from RP4 which increases its stability in Pseudomonas denitrificans, a cobalamin-producing and industrially used bacterium, and (ii) the RK2 oriT has been deleted, leading to a non-mobilizable plasmid.

引用

页码：105 / 108

页数：4

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