OVERPRODUCTION OF THE RECD POLYPEPTIDE SENSITIZES ESCHERICHIA-COLI-CELLS TO GAMMA-RADIATION

被引：10

作者：

BRCICKOSTIC, K ^{[1
]}

STOJILJKOVIC, I ^{[1
]}

SALAJSMIC, E ^{[1
]}

TRGOVCEVIC, Z ^{[1
]}

机构：

[1] RUDJER BOSKOVIC INST, POB 1016, YU-41001 ZAGREB, CROATIA

来源：

MUTATION RESEARCH | 1992年 / 281卷 / 02期

关键词：

ESCHERICHIA-COLI; RECBCD ENZYME; RECD GENE; UV REPAIR; GAMMA-RAY REPAIR;

D O I：

10.1016/0165-7992(92)90046-K

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We investigated DNA metabolism in Escherichia coli cells carrying the multicopy recD+ plasmid (pKI13). In the presence of pKI13, the cellular level of the recD gene product (RecD polypeptide) is amplified at least 60-fold. Overproduction of the RecD polypeptide has no effect on UV repair and conjugational recombination. In contrast, high cellular levels of this polypeptide sensitize wild-type cells to gamma-radiation; also, they increase the rate of radiation-induced DNA degradation. A possible mechanism for the enhancement of gamma-ray-induced killing by large amounts of the RecD polypeptide is discussed.

引用

页码：123 / 127

页数：5

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