DEFINING A NOVEL RIBONUCLEOTIDE REDUCTASE R1 MESSENGER-RNA CIS-ELEMENT THAT BINDS TO AN UNIQUE CYTOPLASMIC TRANS-ACTING PROTEIN

被引：19

作者：

CHEN, FY ^{[1
]}

AMARA, FM ^{[1
]}

WRIGHT, JA ^{[1
]}

机构：

[1] UNIV MANITOBA,MANITOBA INST CELL BIOL,DEPT BIOCHEM & MOLEC BIOL,WINNIPEG R3E 0V9,MB,CANADA

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 22期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/22.22.4796

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribonucleotide reductase is a highly regulated rate-limiting enzyme activity in DNA synthesis, responsible for reducing ribonucleotides to their deoxyribonucleotide forms. Using 3'-end labeled RNA and band-shift and UV cross-linking analyses, we have identified a cis-element, 5'-CAAACUUCS-3', within the 3'-untranslated region of the mammalian ribonucleotide reductase R1 mRNA, which binds a cytoplasmic protein in BALB/c 3T3 mouse cells, to form a 57 kDa RNA-protein complex. Sequence-specific binding was observed, and binding was prevented by several different mutations within the cis-element. We suggest that this cis - trans interaction plays a role in R1 mRNA stability.

引用

页码：4796 / 4797

页数：2

共 19 条

[1] A NOVEL TRANSFORMING GROWTH FACTOR-BETA(1) RESPONSIVE CYTOPLASMIC TRANS-ACTING FACTOR BINDS SELECTIVELY TO THE 3'-UNTRANSLATED REGION OF MAMMALIAN RIBONUCLEOTIDE REDUCTASE-R2 MESSENGER-RNA - ROLE IN MESSAGE STABILITY
AMARA, FM
CHEN, FY
WRIGHT, JA
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (20) : 4803 - 4809
[2] AMARA FM, 1993, J BIOL CHEM, V269, P6709
[3] ATWATER JA, 1990, ANNU REV GENET, V24, P519
[4] REACTIONS AT TERMINI OF TRANSFER-RNA WITH T4 RNA LIGASE
BRUCE, AG
UHLENBECK, OC
[J]. NUCLEIC ACIDS RESEARCH, 1978, 5 (10) : 3665 - 3677
[5] CARTER BZ, 1991, LAB INVEST, V65, P610
[6] 2 GENETIC-LOCI PARTICIPATE IN THE REGULATION BY IRON OF THE GENE FOR THE HUMAN TRANSFERRIN RECEPTOR
CASEY, JL
DIJESO, B
RAO, K
KLAUSNER, RD
HARFORD, JB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (06) : 1787 - 1791
[7] MAMMALIAN RIBONUCLEOTIDE REDUCTASE R1 MESSENGER-RNA STABILITY UNDER NORMAL AND PHORBOL ESTER STIMULATING CONDITIONS - INVOLVEMENT OF A CIS-TRANS-INTERACTION AT THE 3' UNTRANSLATED REGION
CHEN, FY
AMARA, FM
WRIGHT, JA
[J]. EMBO JOURNAL, 1993, 12 (10) : 3977 - 3986
[8] REGULATION OF MAMMALIAN RIBONUCLEOTIDE REDUCTASE R1 MESSENGER-RNA STABILITY IS MEDIATED BY A RIBONUCLEOTIDE REDUCTASE R1 MESSENGER-RNA 3'-UNTRANSLATED REGION CIS-TRANS INTERACTION THROUGH A PROTEIN-KINASE C-CONTROLLED PATHWAY
CHEN, FY
AMARA, FM
WRIGHT, JA
[J]. BIOCHEMICAL JOURNAL, 1994, 302 : 125 - 132
[9] HURTA RAR, 1992, J BIOL CHEM, V267, P7066
[10] HURTA RAR, 1991, J BIOL CHEM, V266, P24097

← 1 2 →