PURIFICATION OF PLANT CALMODULIN BY FLUPHENAZINE-SEPHAROSE AFFINITY CHROMATOGRAPHY

被引：236

作者：

CHARBONNEAU, H

CORMIER, MJ

机构：

[1] Bioluminescence Laboratory, Department of Biochemistry University of Georgia, Athens

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91931-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The calcium-dependent binding of phenothiazine drugs to calmodulin (Levin, R. M. and Weiss, B. (1977) Mol. Pharmacol. 13, 690-697) has been utilized to develop a rapid purification procedure for calmodulin based on fluphenazine-Sepharose affinity chromatography. Calmodulin from plant, a fungus, porcine brain and the coelenterate, Renilla reniformis, were easily purified by the calcium-dependent binding of calmodulin to fluphenazine-Sepharose. © 1979.

引用

页码：1039 / 1047

页数：9

共 33 条

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