ESTIMATION OF FREE TRYPTOPHAN IN PLASMA . A SIMPLIFIED SPECTROFLUOROMETRIC MICROMETHOD

被引：30

作者：

WAPNIR, RA

STEVENSON, JH

机构：

来源：

CLINICA CHIMICA ACTA | 1969年 / 26卷 / 02期

关键词：

D O I：

10.1016/0009-8981(69)90368-4

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Plasma tryptophan can be extracted from spots on filter paper with dilute ethanol and estimated directly by spectrofluorometry in alkaline medium. The use of an inert support simplifies the required deproteinization step permitting good recovery of the free amino acid. This procedure enables the assay of a large number of samples in a short period. Results obtained for normal fasting adults are in agreement with previously published values. © 1969.

引用

页码：203 / +

页数：1

共 9 条

[1] ACKERMANN P, 1949, J LAB CLIN MED, V34, P234
[2] ADREAENS.K, 1967, CLIN CHIM ACTA, V15, P362
[3] DENCKLA WD, 1967, J LAB CLIN MED, V69, P160
[4] DICKMAN SR, 1956, J BIOL CHEM, V220, P957
[5] DUGGAN DE, 1956, J BIOL CHEM, V223, P313
[6] DUNN MS, 1945, J BIOL CHEM, V157, P387
[7] QUANTITATIVE ESTIMATION OF TRYPTOPHAN IN PLASMA AND URINE USING THIN-LAYER CHROMATOGRAPHY (TLC) AND INDUCED FLUORESCENCE
GIBBS, CCJ
SAUNDERS, SJ
SWEENEY, GD
[J]. CLINICA CHIMICA ACTA, 1967, 17 (03) : 317 - &
[8] HARPER HA, 1950, P SOC EXP BIOL MED, V80, P768
[9] HIER SW, 1946, J BIOL CHEM, V163, P129

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