SEQUENCE OF THE STREPTOCOCCUS-PNEUMONIAE BACTERIOPHAGE-HB-3 AMIDASE REVEALS HIGH HOMOLOGY WITH THE MAJOR HOST AUTOLYSIN

被引:55
作者
ROMERO, A [1 ]
LOPEZ, R [1 ]
GARCIA, P [1 ]
机构
[1] CSIC,CTR INVEST BIOL,VELAZQUEZ 144,E-28006 MADRID,SPAIN
关键词
D O I
10.1128/jb.172.9.5064-5070.1990
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have sequenced a DNA fragment containing the pneumococcal bacteriophage HB-3 hbl gene, which codes for the phage lytic amidase. A remarkable nucleotide similarity (87.1%) between the lytA gene, coding for the pneumococcal amidase, the major autolysin of Streptococcus pneumoniae, and the hbl gene was found. This similarity completely disappeared outside the open reading frames coding for both amidases. The hbl gene transformed amidase-deficient strains of S. pneumoniae to the wild-type phenotype, and Southern blotting experiments provided evidence for recombination between donor and recipient genes. A comprehensive evaluation of these and previous results on the peptidoglycan hydrolases of S. pneumoniae and its bacteriophages suggested that recombination mechanisms participate in the evolution of the genes coding for these enzymes.
引用
收藏
页码:5064 / 5070
页数:7
相关论文
共 25 条
  • [1] LYSOGENIC PNEUMOCOCCI AND THEIR BACTERIOPHAGES
    BERNHEIMER, HP
    [J]. JOURNAL OF BACTERIOLOGY, 1979, 138 (02) : 618 - 624
  • [2] CAMPBELL A, 1988, BACTERIOPHAGES, V1, P1
  • [3] CLONING AND SEQUENCING OF AN ESCHERICHIA-COLI GENE, NLP, HIGHLY HOMOLOGOUS TO THE NER GENES OF BACTERIOPHAGE-MU AND BACTERIOPHAGE-D108
    CHOI, YL
    NISHIDA, T
    KAWAMUKAI, M
    UTSUMI, R
    SAKAI, H
    KOMANO, T
    [J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (09) : 5222 - 5225
  • [4] DIAZ E, 1989, J BIOL CHEM, V264, P1238
  • [5] MOLECULAR EVOLUTION OF LYTIC ENZYMES OF STREPTOCOCCUS-PNEUMONIAE AND ITS BACTERIOPHAGES
    GARCIA, E
    GARCIA, JL
    GARCIA, P
    ARRARAS, A
    SANCHEZPUELLES, JM
    LOPEZ, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (03) : 914 - 918
  • [6] GARCIA E, 1985, FEMS MICROBIOL LETT, V29, P77
  • [7] CLONING AND EXPRESSION OF THE PNEUMOCOCCAL AUTOLYSIN GENE IN ESCHERICHIA-COLI
    GARCIA, E
    GARCIA, JL
    RONDA, C
    GARCIA, P
    LOPEZ, R
    [J]. MOLECULAR & GENERAL GENETICS, 1985, 201 (02): : 225 - 230
  • [8] GARCIA E, 1987, STREPTOCOCCAL GENETI, P189
  • [9] OVERPRODUCTION AND RAPID PURIFICATION OF THE AMIDASE OF STREPTOCOCCUS-PNEUMONIAE
    GARCIA, JL
    GARCIA, E
    LOPEZ, R
    [J]. ARCHIVES OF MICROBIOLOGY, 1987, 149 (01) : 52 - 56
  • [10] NUCLEOTIDE-SEQUENCE AND EXPRESSION OF THE PNEUMOCOCCAL AUTOLYSIN GENE FROM ITS OWN PROMOTER IN ESCHERICHIA-COLI
    GARCIA, P
    GARCIA, JL
    GARCIA, E
    LOPEZ, R
    [J]. GENE, 1986, 43 (03) : 265 - 272