HIGH-DENSITY CULTIVATION OF BSK CELLS ON SINTERED ALUMINA CERAMIC FOAM SUPPORT

被引：2

作者：

LEE, DW ^{[1
]}

GRACE, JR ^{[1
]}

CHOW, BKC ^{[1
]}

MACGILLIVRAY, RTA ^{[1
]}

KILBURN, DG ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT CHEM ENGN,BIOTECHNOL LAB,6174 UNIV BLVD,VANCOUVER V6T 1W5,BC,CANADA

来源：

CYTOTECHNOLOGY | 1991年 / 5卷 / 03期

关键词：

HIGH CELL DENSITY; CERAMIC; BHK; PERFUSION SYSTEM; IMMOBILIZATION;

D O I：

10.1007/BF00556293

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A perfusion system which utilizes a porous ceramic core has been tested for the cultivation of transformed BHK cells which produce human transferrin. A design is presented in which cells are immobilized within the porous ceramic particle and are fed by continuous perfusion of batch liquid medium. It was found that more than 5 x 10(9) BHK cells could be supported within the 40 mL ceramic matrix, a ten-fold increase in cell density per unit surface area over the standard roller bottle cultures or a five-fold increase in volumetric cell density over suspension cultures. The cell specific productivity of human transferrin was similar to that observed in suspension culture. The system offers the advantages of significant reduction in serum requirements and the potential for scale-up.

引用

页码：233 / 241

页数：9

共 12 条

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