ASSAY OF SPHINGOLIPID HYDROLASES IN WHITE BLOOD-CELLS WITH LABELED NATURAL SUBSTRATES

被引：74

作者：

SVENNERHOLM, L

HAKANSSON, G

MANSSON, JE

VANIER, MT

机构：

来源：

CLINICA CHIMICA ACTA | 1979年 / 92卷 / 01期

关键词：

D O I：

10.1016/0009-8981(79)90396-6

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Methods were elaborated for the assay on leukocytes of sphingolipid hydrolases with native and artificial substrates. The optimal conditions for the isolation of leukocytes and the preparation of labelled sphingolipids were investigated. The Km and Vmax values for all substrates are given as the specific activities of the sphingolipidhydrolases in normal males and females. It is concluded that leukocytes are the enzyme source of choice for the enzymic diagnosis of neurolipidoses. © 1979.

引用

页码：53 / 64

页数：12

共 19 条

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