RIBOSOMAL-RNA PRECURSORS IN NEURONAL AND GLIAL RAT-BRAIN NUCLEI

被引：14

作者：

STOYKOVA, AS ^{[1
]}

DABEVA, MD ^{[1
]}

DIMOVA, RN ^{[1
]}

HADJIOLOV, AA ^{[1
]}

机构：

[1] BULGARIAN ACAD SCI,INST MOLEC BIOL,BU-1113 SOFIA,BULGARIA

来源：

JOURNAL OF NEUROCHEMISTRY | 1979年 / 33卷 / 04期

关键词：

D O I：

10.1111/j.1471-4159.1979.tb09923.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract– The method of Thompson (1973) for isolation and fractionation of brain nuclei was modified by the introduction of 12mM‐Mg2+ in the isolating media. This technique gives a good yield of pure (85‐90%) neuronal and glial rat brain nuclei, with minimal disruption of nuclei and degradation or processing of nuclear RNA. The RNA/DNA ratio of neuronal nuclei is about 3‐fold higher than that of glial nuclei. Analysis of nucleolar RNA fractions by urea‐agar gel electrophoresis allows the identification of 45S, 41S, 39S, 36S, 32S and 21S pre‐rRNA components. The pattern of nucleolar pre‐rRNA and rRNA species in neuronal and glial nuclei is identical. These results demonstrate the existence in brain nuclei of multiple pre‐rRNA processing pathways qualitatively similar to those observed in other animal tissues. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：931 / &

共 32 条

[1] FRACTIONATION OF NUCLEI FROM BRAIN BY ZONAL CENTRIFUGATION AND A STUDY OF RIBONUCLEIC-ACID POLYMERASE-ACTIVITY IN VARIOUS CLASSES OF NUCLEI [J].

AUSTOKER, J ;

MATHIAS, AP ;

COX, D .

BIOCHEMICAL JOURNAL, 1972, 129 (05) :1139-&

[2] RELEASE OF RNA FROM GOLDFISH BRAIN NUCLEI BY SODIUM DODECYL SULFATE [J].

CASOLA, L ;

AGRANOFF, BW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 30 (03) :262-&

[3]

COOPER HL, 1971, J BIOL CHEM, V246, P5059

[4] INTRANUCLEAR MATURATION PATHWAYS OF RAT-LIVER RIBOSOMAL RIBONUCLEIC-ACIDS [J].

DABEVA, MD ;

DUDOV, KP ;

HADJIOLOV, AA ;

EMANUILOV, I ;

TODOROV, BN .

BIOCHEMICAL JOURNAL, 1976, 160 (03) :495-&

[5] QUANTITATIVE-ANALYSIS OF RAT-LIVER NUCLEOLAR AND NUCLEOPLASMIC RIBOSOMAL RIBONUCLEIC-ACIDS [J].

DABEVA, MD ;

DUDOV, KP ;

HADJIOLOV, AA ;

STOYKOVA, AS .

BIOCHEMICAL JOURNAL, 1978, 171 (02) :367-374

[6] PROCESSING AND MIGRATION OF RIBOSOMAL RIBONUCLEIC-ACIDS IN NUCLEOLUS AND NUCLEOPLASM OF RAT-LIVER NUCLEI [J].

DUDOV, KP ;

DABEVA, MD ;

HADJIOLOV, AA ;

TODOROV, BN .

BIOCHEMICAL JOURNAL, 1978, 171 (02) :375-383

[7]

DUDOV KP, 1976, ANAL BIOCHEM, V76, P250, DOI 10.1016/0003-2697(76)90283-9

[8]

Georgiev G P, 1967, Prog Nucleic Acid Res Mol Biol, V6, P259

[9] ISOLATION AND SOME CHARACTERISTICS OF CELL NUCLEI FROM BRAIN CORTEX OF ADULT CAT [J].

HADJIOLO.AA ;

TENCHEVA, ZS ;

BOJADJIE.AG .

JOURNAL OF CELL BIOLOGY, 1965, 26 (02) :383-&

[10]

Hadjiolov A.A., 1976, Progress Biophys Molec Biol, V31, P95

← 1 2 3 4 →