ELECTROPHORETIC SEPARATION OF S-POMBE CHROMOSOMES IN POLYACRYLAMIDE SOLUTIONS USING A CONSTANT FIELD

被引:21
作者
GUSZCZYNSKI, T
CHRAMBACH, A
机构
[1] Section on Macromolecular Analysis, Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, Bethesda
关键词
D O I
10.1016/0006-291X(91)91396-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous electrophoretic separations of megabase (Mb) sized DNA have been achieved in pulsed electric fields, using agarose gel as a matrix. The present study demonstrates separations of Mb sized DNA due to a retardation of migration in proportion to the concentration of uncrosslinked polyacrylamide of 5×106 molecular weight, using a constant electric field. Potentially, the method should be applicable to large DNA in general, greatly reducing the instrumental complexity of such separations and rendering them compartible with capillary electrophoresis apparatus. © 1991.
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页码:482 / 486
页数:5
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