ELECTROPHORETIC SEPARATION OF S-POMBE CHROMOSOMES IN POLYACRYLAMIDE SOLUTIONS USING A CONSTANT FIELD

被引：21

作者：

GUSZCZYNSKI, T

CHRAMBACH, A

机构：

[1] Section on Macromolecular Analysis, Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, Bethesda

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91396-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous electrophoretic separations of megabase (Mb) sized DNA have been achieved in pulsed electric fields, using agarose gel as a matrix. The present study demonstrates separations of Mb sized DNA due to a retardation of migration in proportion to the concentration of uncrosslinked polyacrylamide of 5×106 molecular weight, using a constant electric field. Potentially, the method should be applicable to large DNA in general, greatly reducing the instrumental complexity of such separations and rendering them compartible with capillary electrophoresis apparatus. © 1991.

引用

页码：482 / 486

页数：5

共 10 条

[1] Benoit H., 1953, J PHYS CHEM-US, V57, P958
[2] BOCEK P, 1991, IN PRESS ELECTROPHOR
[3] LIGHT-SCATTERING STUDIES ON SUPERCOIL UNWINDING
CAMPBELL, AM
JOLLY, DJ
[J]. BIOCHEMICAL JOURNAL, 1973, 133 (02) : 209 - 226
[4] GEMMILL RM, 1991, ADV ELECTROPHORESIS, V4
[5] GUSZCZYNSKI T, 1991, IN PRESS APPL THEOR
[6] ELECTROPHORESIS OF THE NUCLEIC ACIDS
OLIVERA, BM
BAINE, P
DAVIDSON, N
[J]. BIOPOLYMERS, 1964, 2 (03) : 245 - 257
[7] UNIFIED THEORY FOR GEL ELECTROPHORESIS AND GEL FILTRATION
RODBARD, D
CHRAMBACH, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 65 (04) : 970 - +
[8] ESTIMATION OF MOLECULAR RADIUS, FREE MOBILITY, AND VALENCE USING POLYACRYLAMIDE GEL ELECTROPHORESIS
RODBARD, D
CHRAMBACH, A
[J]. ANALYTICAL BIOCHEMISTRY, 1971, 40 (01) : 95 - +
[9] AN ELECTROPHORETIC KARYOTYPE FOR SCHIZOSACCHAROMYCES-POMBE BY PULSED FIELD GEL-ELECTROPHORESIS
SMITH, CL
MATSUMOTO, T
NIWA, O
KLCO, S
FAN, JB
YANAGIDA, M
CANTOR, CR
[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (11) : 4481 - 4489
[10] STELLWAGEN NC, 1987, ADV ELECTROPHORESIS, V1

← 1 →