NEUTRALIZATION OF MYOTOXIC PHOSPHOLIPASES-A2 FROM THE VENOM OF THE SNAKE BOTHROPS-ASPER BY MONOCLONAL-ANTIBODIES

被引：39

作者：

LOMONTE, B

GUTIERREZ, JM

RAMIREZ, M

DIAZ, C

机构：

[1] UNIV COSTA RICA,FAC MICROBIOL,INST CLODOMIRO PICADO,SAN JOSE,COSTA RICA

[2] UNIV COSTA RICA,ECOLE MED,DEPT FISIOL,SAN JOSE,COSTA RICA

[3] LSU,CTR INT INVEST & ADIESTRAMIENTO MED,SAN JOSE,COSTA RICA

来源：

TOXICON | 1992年 / 30卷 / 03期

关键词：

D O I：

10.1016/0041-0101(92)90866-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies.

引用

页码：239 / 245

页数：7

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