CYTOSOLIC LOCALIZATION IN TRANSGENIC PLANTS OF THE ROLC PEPTIDE FROM AGROBACTERIUM-RHIZOGENES

被引：32

作者：

ESTRUCH, JJ

PARETSSOLER, A

SCHMULLING, T

SPENA, A

机构：

[1] MAX PLANCK INST ZUCHTUNGSFORSCH,CARL VON LINNE WEG 10,W-5000 COLOGNE 30,GERMANY

[2] INST AGROQUIM & TECNOL ALIMENTOS,E-46010 VALENCIA,SPAIN

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 17卷 / 03期

关键词：

AGROBACTERIUM-RHIZOGENES; INTRACELLULAR LOCALIZATION; ROLC PEPTIDE; WESTERN BLOT ANALYSIS;

D O I：

10.1007/BF00040654

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rolC gene of Agrobacterium rhizogenes codes for a peptide with an apparent molecular weight of approximately 20 kDa. Immunolocalization of the rolC peptide, in leaves of transgenic plants which are genetic mosaics for the expression of the rolC gene, is restricted to the phenotypically altered sectors. Subcellular fractionation of homogenates from 35S-rolC transgenic leaves shows the cytosolic localization of the rolC peptide.

引用

页码：547 / 550

页数：4

共 13 条

[1]

BERGMEYER HU, 1984, METHODS ENZYMATIC AN, V4

[2]

BERGMEYER HU, 1984, METHODS ENZYMATIC AN, V2

[3]

BERGMEYER HU, 1984, METHODS ENZYMATIC AN, V1

[4] A RAPID, SENSITIVE METHOD FOR DETECTION OF ALKALINE-PHOSPHATASE CONJUGATED ANTI-ANTIBODY ON WESTERN BLOTS [J].