STRUCTURE OF THE GENE ENCODING ANCHORIN-CII (CHICK ANNEXIN V)

被引:14
作者
FERNANDEZ, MP
FERNANDEZ, MR
MORGAN, RO
机构
[1] CHILDRENS NATL MED CTR,WASHINGTON,DC 20010
[2] GEORGE WASHINGTON UNIV,WASHINGTON,DC 20010
关键词
GENOMIC DNA; CALCIUM-BINDING PROTEIN; COLLAGEN-BINDING PROTEIN; PHOSPHOLIPID-BINDING PROTEIN; LIPOCORTIN V; CIS-REGULATORY ELEMENTS;
D O I
10.1016/0378-1119(94)90568-1
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Anchorin CII (annexin V) was first characterized as a collagen-binding protein and later identified as the chick homologue of human endonexin II, a member of the annexin gene family. Its gene (anx5) structure and sequence have been investigated to provide insight into the evolution and regulation of this important protein, and to elucidate its putative role in signal transduction and cellular differentiation. Four chick genomic clones encoding anchorin CII were isolated and characterized by restriction analysis, Southern blotting and sequencing. The anchorin CII-encoding gene spans about 24 kb and consists of 13 exons ranging in length from 50 to 561 bp, interrupted by 12 introns of 94 bp to 7 kb. Its promoter sequence contained no TATA box, but did display a high GS C content and multiple Spl-binding sites typical of 'housekeeping' genes. Potential binding sites for transcription factors in the 5'-upstream region are consistent with regulation of anx5 expression by mitogens, oncoproteins, steroids and possibly metals. Genomic Southern blotting confirmed that chick anx5 is present as a single-copy gene.
引用
收藏
页码:179 / 186
页数:8
相关论文
共 39 条
[1]   STRUCTURE AND CHROMOSOME ASSIGNMENT OF THE MURINE P36 (CALPACTIN-I HEAVY-CHAIN) GENE [J].
AMIGUET, P ;
DEUSTACHIO, P ;
KRISTENSEN, T ;
WETSEL, RA ;
SARIS, CJM ;
HUNTER, T ;
CHAPLIN, DD ;
TACK, BF .
BIOCHEMISTRY, 1990, 29 (05) :1226-1232
[2]   AMINO-ACID-SEQUENCE ANALYSIS OF THE ANNEXIN SUPERGENE FAMILY OF PROTEINS [J].
BARTON, GJ ;
NEWMAN, RH ;
FREEMONT, PS ;
CRUMPTON, MJ .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 198 (03) :749-760
[3]   EVOLUTION OF EF-HAND CALCIUM-MODULATED PROTEINS .5. THE GENES ENCODING EF-HAND PROTEINS ARE NOT CLUSTERED IN MAMMALIAN GENOMES [J].
BERCHTOLD, MW .
JOURNAL OF MOLECULAR EVOLUTION, 1993, 36 (05) :489-496
[4]   STRUCTURE OF CHICKEN ANNEXIN-V AT 2.25-ANGSTROM RESOLUTION [J].
BEWLEY, MC ;
BOUSTEAD, CM ;
WALKER, JH ;
WALLER, DA .
BIOCHEMISTRY, 1993, 32 (15) :3923-3929
[5]   TRANSCRIPTIONAL INITIATION IS CONTROLLED BY UPSTREAM GC-BOX INTERACTIONS IN A TATAA-LESS PROMOTER [J].
BLAKE, MC ;
JAMBOU, RC ;
SWICK, AG ;
KAHN, JW ;
AZIZKHAN, JC .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) :6632-6641
[6]  
BRASELMANN S, 1992, J CELL SCI S, V16, P97
[7]  
BROWNING JL, 1990, PROG CLIN BIOL RES, V349, P27
[8]   THE ANNEXIN FAMILY OF CALCIUM-BINDING PROTEINS [J].
BURGOYNE, RD ;
GEISOW, MJ .
CELL CALCIUM, 1989, 10 (01) :1-10
[9]  
FERNANDEZ MP, 1988, J BIOL CHEM, V263, P5921
[10]  
FERNANDEZ MP, 1990, J BIOL CHEM, V265, P8344