MEASUREMENT OF SOLVOLYZABLE ESTERS OF ANDROSTERONE AND DEHYDROISOANDROSTERONE IN HUMAN PLASMA

被引：17

作者：

ROSENFELD, RS

HELLMAN, L

机构：

[1] Institute for Steroid Research, the Division of Neoplastic Medicine, Montefiore Hospital, New York

来源：

STEROIDS | 1969年 / 14卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0039-128X(69)80075-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been presented for the determination of the solvolyzable esters of androsterone and dehydroisoandrosterone in human plasma. The procedure involves the addition of tracer amounts of [4-14C] dehydroisoandrosterone and [3-3H] androsterone to plasma after extraction of the free steroids followed by protein precipitation, solvolysis, defatting, alumina chromatography, and thin layer chromatography. Quantitation is done by gas-liquid chromatography of the trimethylsilyl ethers. Replicate analyses of two large pools of adult human plasma gave values of 38 ± 2 μg and 47 ± 3 μg for androsterone and 147 ± 16 μg and 152 ± 10 μg for dehydroisoandrosterone as solvolyzable esters. Levels in 31 females and 24 males are reported. The procedure is applicable for routine laboratory use. © 1969.

引用

页码：675 / +

页数：1

共 14 条

[1]

BAULIEU EE, 1963, RECENT PROGR HORMONE, V19, P306

[2]

BURSTEIN S, 1958, J BIOL CHEM, V233, P331

[3] STUDIES WITH ANDROSTERONE-3-3H,4-14C [J].