HYDROXYL RADICAL PRODUCTION IN A PURIFIED NADPH-CYTOCHROME-C (P-450) REDUCTASE SYSTEM

被引：77

作者：

LAI, CS ^{[1
]}

GROVER, TA ^{[1
]}

PIETTE, LH ^{[1
]}

机构：

[1] UNIV HAWAII,PBRC,CANC RES CTR HAWAII,HONOLULU,HI 96813

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 193卷 / 02期

关键词：

D O I：

10.1016/0003-9861(79)90042-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using the spin trap, 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) we have demonstrated that hydroxyl radicals are generated indirectly from purified preparations of rat liver microsomal NADPH-cytochrome c (P-450) reductase during NADPH oxidation. Hydroxyl radical formation is completely inhibited by p-chloromercuribenzoate, but not by metyrapone. In addition, hydroxyl radical DMPO adduct formation is blocked by added linolenic acid which, in turn, is peroxidatively degraded into malondialdehyde, suggesting that hydroxyl radicals formed from purified NADPH-cytochrome c (P-450) reductase are capable of initiating lipid peroxidation. A mechanism for the indirect production of hydroxyl radicals from NADPH-cytochrome P-450 reductase is discussed. © 1979.

引用

页码：373 / 378

页数：6

共 45 条

[1] SUPEROXIDE ANION INVOLVEMENT IN NBT REDUCTION CATALYZED BY NADPH-CYTOCHROME RHO-450 REDUCTASE - PITFALL [J].