MYELOID CALCIUM-BINDING PROTEINS - EXPRESSION IN THE DIFFERENTIATED HL-60 CELLS AND DETECTION IN SERA OF PATIENTS WITH CONNECTIVE-TISSUE DISEASES

被引:44
作者
KURUTO, R
NOZAWA, R
TAKEISHI, K
ARAI, K
YOKOTA, T
TAKASAKI, Y
机构
[1] UNIV SHIZUOKA, SCH FOOD & NUTR SCI, GENET ENGN LABS, OYA, SHIZUOKA 422, JAPAN
[2] JUNTENDO UNIV, SCH MED, DEPT BACTERIOL, BUNKYO KU, TOKYO 113, JAPAN
[3] JUNTENDO UNIV, SCH MED, DEPT MED, BUNKYO KU, TOKYO 113, JAPAN
关键词
D O I
10.1093/oxfordjournals.jbchem.a123257
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A differentiation antigen induced in 1,25-dihydroxyvitamin D5 (VD3)-treated HL-60 cells was identified as being comprised of the myeloid calcium binding proteins CaBP-p8 and -pl4 by determining its amino acid and DNA sequence. Northern blot analysis using a DNA fragment of the gene encoding p 14 as a probe indicated that the gene was not expressed in undifferentiated HL-60 cells but transcribed starting on day 1 after VD5 treatment. The level of p 14 mRNA reached a peak on day 2, then declined, and little mRNA remained on day 10, indicating that the p14 gene is activated once and then inactivated during HL-60 differentiation due to VD3. In contrast, thymidylate synthase (TSase) mRNA was present in undifferentiated HL-60 cells but disappeared quickly after VD3 treatment. Both p8 and p14 of CaBP were found at elevated levels in sera of some patients with connective tissue diseases [highly elevated in rheumatoid arthritis (RA), Sjogren's syndrome (SjS), systemic lupus erythematosus (SLE), and progressive systemic sclerosis (PSS), and moderately in polymyositis or dermatomyositis (PM/DM) and mixed connective tissue disease (MCTD)]. These results were in sharp contrast with the finding that p14 is always at a highly elevated level but little p8 is present in the sera of cystic fibrosis (CF) patients [Bruggen et al. (1988) Nature 331, 570). © 1990 Copyright, 1990 by the Journal of Biochemistry.
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页码:650 / 653
页数:4
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