DOUBLE-LABEL AUTO-RADIOGRAPHY FOR COMPARISON OF COMPLEX PROTEIN MIXTURES AFTER GEL-ELECTROPHORESIS

被引：63

作者：

MCCONKEY, EH

机构：

[1] Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90551-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparison of complex protein mixtures of high-resolution, 2-dimensional polyacrylamide gel electrophoresis is simplified by coelectrophoresis of 3H-labeled and 14C-labeled preparations, followed by 2-step autoradiography. Both qualitative and quantitative comparisons are possible. Applications to genetics, molecular evolution and metabolism are described.

引用

页码：39 / 44

页数：6

共 16 条

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[7] MCCONKEY EH, UNPUBLISHED
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[9] ANALYSIS OF HELA-CELL HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE MUTANTS AND REVERTANTS BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS - EVIDENCE FOR SILENT GENE ACTIVATION
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[10] OFARRELL PH, 1975, J BIOL CHEM, V250, P4007

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