NOVEL METHOD FOR MATRIX-ASSISTED LASER MASS-SPECTROMETRY OF PROTEINS

被引：81

作者：

ZHAO, S

SOMAYAJULA, KV

SHARKEY, AG

HERCULES, DM

HILLENKAMP, F

KARAS, M

INGENDOH, A

机构：

[1] UNIV PITTSBURGH,DEPT CHEM,PITTSBURGH,PA 15260

[2] UNIV MUNSTER,INST MED PHYS,W-4400 MUNSTER,GERMANY

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 05期

关键词：

D O I：

10.1021/ac00005a012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method is demonstrated for obtaining laser mass spectra of proteins by using a nitrobenzyl alcohol matrix (NBA), consisting of nitrobenzyl alcohol, methanol, and water in a ratio of 3:2:1, and by using fibrous material as the substrate. Selected proteins were measured up to mass 97 400. The detection limit when using the NBA matrix is demonstrated to be 5 fmol for a single-shot spectrum of albumin. Mixtures of up to six proteins were analyzed and their molecular weights estimated by centroid analysis. Supression effects were observed when pepsin is present in a mixture of peptides.

引用

页码：450 / 453

页数：4

共 16 条

[1] Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P432, DOI 10.1002/rcm.1290031207
[2] Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P233, DOI 10.1002/rcm.1290030708
[3] BEAVIS RC, 1989, 37TH P ASMS C MASS S, P1186
[4] LAMMA-1000, A NEW LASER MICROPROBE MASS ANALYZER FOR BULK SAMPLES
HEINEN, HJ
MEIER, S
VOGT, H
WECHSUNG, R
[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1983, 47 (JAN): : 19 - 22
[5] HILLENKAMP F, 1989, ADV MASS SPECTROM, V11, P354
[6] HILLENKAMP F, 1989, 37TH P ASMS C MASS S, P1168
[7] HILLENKAMP F, IN PRESS 2ND P INT S
[8] ULTRAVIOLET-LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF FEMTOMOLAR AMOUNTS OF LARGE PROTEINS
KARAS, M
INGENDOH, A
BAHR, U
HILLENKAMP, F
[J]. BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1989, 18 (09): : 841 - 843
[9] LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF PROTEINS OF MASS 100 000 TO 250 000 DALTON
KARAS, M
BAHR, U
INGENDOH, A
HILLENKAMP, F
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1989, 28 (06): : 760 - 761
[10] MATRIX-ASSISTED ULTRAVIOLET-LASER DESORPTION OF NONVOLATILE COMPOUNDS
KARAS, M
BACHMANN, D
BAHR, U
HILLENKAMP, F
[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1987, 78 : 53 - 68

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