CAPILLARY ELECTROPHORESIS USING AIR AND HELIUM AS COOLING FLUIDS

被引：11

作者：

CIFUENTES, A ^{[1
]}

KOK, WT ^{[1
]}

POPPE, H ^{[1
]}

机构：

[1] UNIV AMSTERDAM,AIMS,ANALYT CHEM LAB,1018 WV AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1995年 / 7卷 / 04期

关键词：

CAPILLARY ELECTROPHORESIS; TEMPERATURE CONTROL; HEAT TRANSFER; HELIUM GAS COOLING;

D O I：

10.1002/mcs.1220070409

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A comparative study on the properties of helium and air as thermostatting fluids in Capillary Electrophoresis (CE) has been carried out. Equations that relate the properties of fluids to their control of the temperature of the capillary are shown. Results from theory and experiments are compared under different cooling conditions, i.e., natural and forced gas convection, using air and helium. Experimental results are qualitatively in good agreement with those predicted by theory. The better thermostatting capability of helium compared to air is demonstrated by an improved efficiency in real separations and a lower degree of denaturation of a thermolabile protein during its purification by CE. (C) 1995 John Wiley & Sons, Inc.

引用

页码：365 / 374

页数：10

共 23 条

[1] UNSTEADY HEAT-TRANSFER IN CAPILLARY ZONE ELECTROPHORESIS .1. A MATHEMATICAL-MODEL [J].