ENTAMOEBA-HISTOLYTICA - MECHANISM OF SURFACE-RECEPTOR

被引：37

作者：

ESPINOSACANTELLANO, M ^{[1
]}

MARTINEZPALOMO, A ^{[1
]}

机构：

[1] INST POLITECN NACL, CTR INVEST & ESTUDIOS AVANZADOS, DEPT EXPTL PATHOL, MEXICO CITY 07000, DF, MEXICO

来源：

EXPERIMENTAL PARASITOLOGY | 1994年 / 79卷 / 03期

关键词：

ENTAMOEBA HISTOLYTICA; AMEBIASIS; PROTOZOA; PARASITE; CAPPING; ANTIBODIES; CONCANAVALIN A; FLUORESCEIN ISOTHIOCYANATE; BOVINE SERUM ALBUMIN; P-HYDROXYMERCURIBENZOATE; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; PHOSPHATE-BUFFERED SALINE;

D O I：

10.1006/expr.1994.1104

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Binding of antibodies to the surface of the parasitic protozoan Entamoeba histolytica induces capping and release of antigen-antibody complexes, in a process that may be an effective means of evading the host immune response. To analyze the mechanism of capping in E. histolytica trophozoites, optimal conditions were determined for cap induction by sera from patients with amebic liver abscess, by polyclonal anti-amebic antibodies, and by concanavalin A. Cocapping of actin and myosin was documented by immunofluorescent staining of capped amebas using phalloidin, monoclonal anti-actin antibodies, and polyclonal anti-myosin antibodies. Immunoblots confirmed the presence of actin and myosin in isolated caps. These observations demonstrate that amebic cytoskeletal components participate in E. histolytica capping of surface receptors. (C) 1994 Academic Press, Inc.

引用

页码：424 / 435

页数：12

共 33 条

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