PYROSTATIN-A AND PYROSTATIN-B, NEW INHIBITORS OF N-ACETYL-BETA-D-GLUCOSAMINIDASE, PRODUCED BY STREPTOMYCES SP SA-3501

被引：22

作者：

AOYAMA, T ^{[1
]}

KOJIMA, F ^{[1
]}

IMADA, C ^{[1
]}

MURAOKA, Y ^{[1
]}

NAGANAWA, H ^{[1
]}

OKAMI, Y ^{[1
]}

TAKEUCHI, T ^{[1
]}

AOYAGI, T ^{[1
]}

机构：

[1] INST MICROBIAL CHEM,SHINAGAWA KU,TOKYO 141,JAPAN

来源：

JOURNAL OF ENZYME INHIBITION | 1995年 / 8卷 / 04期

关键词：

N-ACETYL-BETA-D-GLUCOSAMINIDASE; INHIBITOR; PYROSTATIN; STREPTOMYCES; MARINE MICROBE;

D O I：

10.3109/14756369509020129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyrostatins A and B, new inhibitors of N-acetyl-beta-D-glucosaminidase (GlcNAc-ase), have be en purified from the culture broth of Streptomyces sp. SA-3501 isolated from a marine environment. They were purified by chromatography on Dowex 50W silica gel and Capcell Pak C-18(HPLC) followed treatment with active carbon and then isolated as white powders. The structures of pyrostatins A and B were determined by NMR studies to be 4-hydroxy-2-imino-1-methylpyrrolidine-5-carboxylic acid and 2-imino-1-methylpyrrolidine-5-carboxylic acid, respectively. They were competitive with the substrate, and the inhibition constants(K-i) of pyrostatins A and B were 1.7 x 10(-6) M and 2.0 x 10(-6) M respectively.

引用

页码：223 / 232

页数：10

共 18 条

[1] CHARACTERIZATION OF BETA-HEXOSAMINIDASE FROM LIVER AND SERA OF DIABETIC-PATIENTS AND CONTROLS [J].