DETERMINATION OF MAGNESIUM BINDING TO MACROMOLECULES

被引：5

作者：

OLSON, DL ^{[1
]}

DEERFIELD, DW ^{[1
]}

BERKOWITZ, P ^{[1
]}

HISKEY, RG ^{[1
]}

PEDERSEN, LG ^{[1
]}

机构：

[1] UNIV N CAROLINA, DEPT BIOCHEM, CHAPEL HILL, NC 27514 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1987年 / 160卷 / 02期

关键词：

D O I：

10.1016/0003-2697(87)90077-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An equilibrium dialysis technique for examining magnesium binding to macromolecules is described. The technique is used to determine the binding constants of magnesium to human prothrombin. This procedure should be of great utility for many biochemical systems which exhibit magnesium affinity.

引用

页码：468 / 470

页数：3

共 19 条

[1] Adair GS, 1925, J BIOL CHEM, V63, P529
[2] QUIN-2 - THE DISSOCIATION-CONSTANTS OF ITS CA-2+ AND MG-2+ COMPLEXES AND ITS USE IN A FLUORIMETRIC METHOD FOR DETERMINING THE DISSOCIATION OF CA-2+-PROTEIN COMPLEXES
BRYANT, DTW
[J]. BIOCHEMICAL JOURNAL, 1985, 226 (02) : 613 - 616
[3] CALMODULIN PLAYS A PIVOTAL ROLE IN CELLULAR-REGULATION
CHEUNG, WY
[J]. SCIENCE, 1980, 207 (4426) : 19 - 27
[4] DEERFIELD DW, 1986, J BIOL CHEM, V261, P4833
[5] DEERFIELD DW, UNPUB
[6] Hughes M.N., 1981, INORGANIC CHEM BIOL, V2nd
[7] JOHNSON AE, 1983, J BIOL CHEM, V258, P5554
[8] PURIFICATION AND CHARACTERIZATION OF HUMAN FACTOR II
KISIEL, W
HANAHAN, DJ
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 304 (01) : 103 - 113
[9] KLOTZ IM, 1986, INTRO BIOMOLECULAR E, P114
[10] The constitution and fundamental properties of solids and liquids Part I Solids
Langmuir, I
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1916, 38 : 2221 - 2295

← 1 2 →