HAPLOID PLANTS FROM POLLEN GRAINS

被引:1164
作者
NITSCH, JP
NITSCH, C
机构
[1] Laboratoire de Physiologie Pluricellulaire, Centre National de la Recherche Scientifique, 91, Gif-sur-Yvette
关键词
D O I
10.1126/science.163.3862.85
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like, structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.
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页码:85 / &
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