USE OF MINI-COLUMNS PACKED WITH ACID-WASHED FLORISIL FOR THE RAPID SEPARATION OF A, E AND F SERIES PROSTAGLANDINS

被引:8
作者
BANSCHBACH, MW
LOVE, PK
机构
[1] Department of Biochemistry, Molecular Biology The Louisiana State University Medical Center School of Medicine in Shreveport, Shreveport
来源
PROSTAGLANDINS | 1979年 / 17卷 / 02期
关键词
D O I
10.1016/0090-6980(79)90038-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new procedure was developed for the rapid isolation of the A, E and F series prostaglandins from plasma extracts. This new procedure involves the use of mini-columns packed with acid-washed Florisil to rapidly separate the A, E and F series prostaglandins. These new mini-columns give flow rates of around 1 ml/min. and they generate a well-resolved chromatographic pattern while at the same time producing good recoveries for [3H]-labeled prostaglandins of the A, E and F series. Using these new mini-columns, large numbers of plasma extracts can be processed in a short period of time. © 1979.
引用
收藏
页码:193 / 200
页数:8
相关论文
共 6 条
  • [1] CARROLL KK, 1961, J LIPID RES, V2, P135
  • [3] CARROLL KK, 1976, LIPID CHROMATOGRAPHI, V1, P173
  • [4] CHROMATOGRAPHIC PATTERNS OF PROSTAGLANDINS USING DIFFERENT LOTS OF SILICIC-ACID
    KIBBEY, WE
    BRONN, DG
    MINTON, JP
    [J]. PROSTAGLANDINS, 1977, 13 (06): : 1023 - 1032
  • [5] SHAW J E, 1969, Methods of Biochemical Analysis, V17:, P325, DOI 10.1002/9780470110355.ch8
  • [6] INFLUENCE OF PH ON EXTRACTION OF PGE2 AND PGF2ALPHA FROM RABBIT PLASMA
    VALENZUELA, G
    HARPER, MJK
    [J]. PROSTAGLANDINS, 1976, 12 (03): : 377 - 381