A PROTEIN WITH HOMOLOGY TO THE C-TERMINAL REPEAT SEQUENCE OF OCTOPUS RHODOPSIN AND SYNAPTOPHYSIN IS A MEMBER OF A MULTIGENE FAMILY IN DICTYOSTELIUM-DISCOIDEUM

被引：23

作者：

NOEGEL, AA ^{[1
]}

GERISCH, G ^{[1
]}

LOTTSPEICH, F ^{[1
]}

SCHLEICHER, M ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

FEBS LETTERS | 1990年 / 266卷 / 1-2期

关键词：

Actin binding protein; Actin-membrane interaction; Dictyostelium; Octopus rhodopsin; Synaptophysin;

D O I：

10.1016/0014-5793(90)81521-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies were raised against a protein with a molecular mass of 24 kDa that has been described as a membrane-associated, actin binding protein from Dictyostelium discoideum [(1985) J. Cell Biol. 100, 727-735]. Using these monoclonal antibodies we isolated from a λgt11 expression library cDNA clones coding for this protein. The cDNA deduced amino acid sequence revealed the presence of an unusual carboxy-terminus which has homologies to the C-termini of Octopus rhodopsin and synaptophysin. This part of the protein sequence contains 5 direct repeats with the motif GYP (P)Q(P). Southern and Northern blots showed that this sequence is present in a series of Dictyostelium genes transcribed in all stages of development. © 1990.

引用

页码：118 / 122

页数：5

共 27 条

[1] RAPID METHOD FOR ISOLATION OF L-CELL SURFACE MEMBRANES USING AN AQUEOUS 2-PHASE POLYMER SYSTEM [J].