SECONDARY STRUCTURES OF TETRAHYMENA THERMOPHILA RIBOSOMAL-RNA IVS SEQUENCE INVOLVED IN ITS SELF-SPLICING REACTIONS - A NEW COMPUTER-ANALYSIS

The secondary structures of Tetrahymena thermophila rRNA IVS sequence involved in the self-splicing reactions, are theoretically investigated with a refined computer method previously proposed, able to select a set of the deepest free energy RNA secondary structures under contraints of model hypotheses and experimental evidences. The secondary structures obtained are characterized by the close proximity of self-reactions sites and account for double mutations experiments, and differential digestion data.